Interaction of Stoned and Synaptotagmin in Synaptic Vesicle Endocytosis

Interaction of stoned and synaptotagmin in synaptic vesicle endocytosis.

The Drosophila dicistronic stoned locus encodes two distinctive presynaptic proteins, Stoned A (STNA) and Stoned B (STNB); STNA is a novel protein without homology to known synaptic proteins, and STNB contains a domain with homology to the endocytotic protein AP50. Both Stoned proteins colocalize precisely with endocytotic proteins including the AP2 complex and Dynamin in the "lattice network" ...

Dissecting Synaptic Vesicle Endocytosis

Synaptic vesicle endocytosis.

Exocytosis of synaptic vesicles is followed rapidly by reinternalization and recycling of their membranes. Recent studies have confirmed the key role of clathrin-mediated endocytosis in synaptic vesicle reformation and have identified new proteins that participate in this process. In addition, growing evidence suggests that lipids, primarily phosphoinositides, play an important role in synaptic...

Living synaptic vesicle marker: synaptotagmin-GFP.

Synapses are the site of chemical communication between neurons and between neurons and muscles. The synaptic vesicle (SV) is a prominent presynaptic organelle which contains chemical neurotransmitters and fuses with the plasma membrane to mediate neurotransmission. There are about 50 or so synaptic proteins which are either integral vesicle membrane proteins (e.g., synaptotagmin, syt; and syna...

Human stoned B interacts with AP-2 and synaptotagmin and facilitates clathrin-coated vesicle uncoating.

Synaptic vesicle biogenesis involves the recycling of synaptic vesicle components by clathrin-mediated endocytosis from the presynaptic membrane. stoned B, a protein encoded by the stoned locus in Drosophila melanogaster has been shown to regulate vesicle recycling by interacting with synaptotagmin. We report here the identification and characterization of a human homolog of stoned B (hStnB). H...